A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

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Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and Clothing - Mens Tops - Jackets accordingly are targets for rice yield improvement.In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1.Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system.Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n.The frequency of dep1 was obviously higher than that of S5-n.

The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable.It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle Gift Card allele dep1 to breed super rice varieties using indica and japonica heterosis.